When peaks were detected, you can assign Substance to them.
The view is made of three main parts:
Profiles view (left)
Substances view (middle)
Tracks view (right)
The Profiles viewer is only in 2D mode here. The Zoom function can be helpful to quickly see peaks in details without changing of view.
When zooming, the substance position overlay may not be placed correctly, go back to 1:1 display to see it correctly.
When a substance is selected, an overlay is displayed on the profiles viewer, corresponding the ΔRf above and below the found Rf of the substance.
You can change the ΔRf of the substance by dragging one of the dashed lines or one of the triangle:
Only the area between bounds is displayed.
Even after being manually adjusted, ΔRf is automatically set to the minimal value possible for containing all the peaks.
Each substance is displayed by the way of a thumb (placed on the corresponding Rf):
The substance name is written and the wavelength used for her calibration.
You can do the following for the substance by interacting with the thumb:
- Change the Rf of the substance
By dragging the thumb.
- Select the used wavelength
By selecting in the combo-box the new wavelength (if more wavelength are available in the current TLC step).
- Manually assign peak to the substance
When toggle on, you can select one peak per track. You can also un-assign a peak by clicking on it. When you have finished the assignment for all tracks, toggle off the button.
- Display the assignment
By clicking on the eye button the overlay on profiles view and tracks view is displayed. The column of the substance is also selected in the Substance Table.
Even after being manually adjusted, Rf is automatically set to mean Rf value of the peaks. ΔRf is then adjusted. Only manual assign will not changes theses values.
Manual assign give you the choice to select any peak: it is possible to have two substances crossing across the plate, which may or may not be correct.
All active tracks are displayed. No photo is used, but a pseudo track in generated from the profile:
Right of the track number, the type of sequence is displayed, for reference and for sample. The icons have tooltips displaying the application in details.
On each track, all detected peaks are displayed by a small rectangle at the max Rf (peak start and end aren’t displayed).
The color of the peak rectangle correspond to:
Grey: peak without an assigned substance
Green: peak with an assigned substance
Yellow: assigned peak of the selected substance
When a substance is selected, an overlay is displayed on all tracks, the white band showing the ΔRf, and the dashed red line the Rf value used for the substance.
If you select a track (by clicking on it), only its profile would be displayed in the Profiles view. Only one track can be selected at the time.
The toolbar give access to some advanced functionalities:
Show all tracks: if only one profile was displayed in Profiles view, it will display again all profile
Reset assignment: restart the automatic substance assignment
Add substance: you will be asked to click on a peak, a new substance will be automatically assigned to it
Export the substance assignment data as CSV file