defined the substances and their concentrations in the Definition tab and
assigned the substances to their corresponding peaks in the Subst. Assign. tab,
use the Calibration tab to configure and see the regression functions used to deduct the quantity of each substance in the sample applications, based on the reference applications.
For each vial applied as reference with a given volume on a track, the substance concentration the vial contains (as defined in the Definition tab) gives the quantity X of substance applied. Depending on the current calibration parameters, the height or the area of the peak is considered as Y value to produce a reference point of coordinates (X,Y). 2 reference vials applied with the same volume are considered as replicas, because in errorless conditions the 2 applications should produce the same reference point. The regression function Y=F(X) is computed by taking each center of gravity of reference points’ replicas as input data, and by applying the algorithm corresponding to the calibration mode chosen.
For each vial applied as sample having a peak assigned to the substance being quantified, the peak height or area gives an Y value, and its corresponding quantity X is computed by resolving X=InvF(Y) where InvF is the inverse of the function F.
In case of reference overspotting of the same vial, the sum of the quantities produce the reference point. When mixing reference and sample overspots of vials containing a quantified substance, the reference and sample quantities applied on the track produce 2 different points (one reference point and one sample point).
First, select a substance in the Overview panel on the left to its calibration data, displayed in the main area.
The calibration data view is made of 2 elements:
Height/Area views (top)
Calibration function view (bottom)
In this area, 2 parameters can be configured:
The calibration type (Height or Area, understood as “height/area of the peaks”).
The calibration mode, by choosing between the list of available regression functions.
This 2 parameters can be configured in the Definition tab too.
This area also displays the regression function itself, its standard deviation (this is an indication of deviation between the regression function and the reference points used to compute it).
In the regression function, x is always expressed in grams (g), because it has to be unit-independent. The display of the curve in the bottom area does not follow this rule, in order to display more user-friendly values. This behavior is new in visionCATS 2.0 SP1.
Please note that many situations prevent the calibration from giving results:
There aren’t any reference or sample assignments (sample points are kept only in the range of the reference points quantities, except for the Linear-1 mode) for this substance,
There aren’t enough replicas group (Linear-1 requires 1 replicas group, Linear-2 and Mime-1 require 2 replicas groups, Polynomial and Mime-2 require 3 replicas groups),
The regression function computed is not strictly increasing in the range of the reference quantities,
In case of Mime regressions, the iterative process diverges.
Calibration function view¶
This area gives a visual feedback of the calibration by showing the calibration curve, the reference points and the sample points in the same chart. The chart zoom can be controlled by using the mouse wheel or a selection rectangle. When hovering the mouse on a reference or sample point, the tooltip displays the details of its associated data:
The corresponding track,
The detail of the vial application(s) corresponding to the point, with the vial ID, description and volume applied,
The exact coordinates of the point.