The main goal of the Evaluation functionality is to perform quantitative evaluations of substances:
You first define the substances you want to search, and the concentration of those substances in the reference vials of the analysis. This process is called the Definition. The application volume of each vial on the plate, associated with the concentration, gives the quantity applied for each reference.
Then you configure (if needed) the peak detection, based on profiles issued from the execution of a scanner step or generated from images taken during a Visualizer step. This process is called the Integration.
When the substances and the peaks are defined, you associate them. This process is called the Substance Assignment. For a substance assigned to a peak belonging to a reference application, the height or the area of the peak is associated with the quantity applied.
For a given substance, the set of couples (quantity applied, height or area of the peak) for references applications are projected on an XY plan, and a regression algorithm is used to find an appropriate regression curve for these reference points. Peaks coming from tracks applied as sample also have a height (and an area). The regression algorithm calculates the quantity of the substance applied in the sample. This process is called the Calibration.
In the end, the Results are displayed. The quantity of substance applied in each sample application computed during the calibration, associated with the volume applied of the vial, gives the estimated concentration of each searched substance in each sample vial concerned. This is the main result of the quantitative evaluation.
A method/analysis can contain one or more evaluations, each one with different parameters. To add an evaluation, click on the + tab of the analysis:
A maximum of five evaluations can be added to one analysis, and each evaluation is identified by its own tab:
Each evaluation has several steps, from definition to results. You can navigate between steps of one evaluation by clicking on the corresponding button:
The different steps are detailed in the following section: