The main goal of the Spectrum functionality is to perform and analyze measurements for a wavelength range at given positions on the plate. visionCATS uses the following process to perform spectrum measurements:
First, define a scanner step (Scanner 3 or Scanner 4) in the HPTLC Steps tab after an existing (non-clean) documentation step. In the Chromatography tab, choose Spectrum as Scanner type in the settings of the scanner step and configure the corresponding spectrum parameters. You can define this step in a method or an analysis just like any other step. A previous documentation step is required because you’ll have to define the positions of the spectrum based on existing profile data coming either from Visualizer generated profiles or from Scanner profile data.
When executing the spectrum scan step, the Positioning step is used to define the positions of the substances to perform the spectrum for.
When the desired positions are defined, the spectrum scanner step can be executed.
The execution of the spectrum scan step produces the spectrum data itself that you can browse for further analysis. Purity data is also shown (when the option was activated in the scanner step parameters). Optionally, you can then define the Correlation between tracks.
Define new spectrum scan steps and executing them is only possible on visionCATS installations having the Spectrum scanning option.
Each analysis has an unique Spectrum tab for all spectrum scan steps defined. This tab contains the following steps:
The different steps are detailed in the following section: