After having detected the peaks during the Integration, each peak you are interested in has to be assigned to a Substance. This association realizes the link between the track profile data measured and the substances to be quantified in the current evaluation. In order for the quantitative evaluation to be successful, remember that an assignment of a peak with a substance means that the substance is detected on the track of this peak. The track’s reference vials applied (multiple applications possible in case of overspots), as defined in the Definition tab, are eventually associated with a concentration and a purity for the substance being applied. The Calibration will only be able to find a reference calibration point for this substance if this association exists.
The Overview section displays the wavelength(s)/illumination(s) selected during the Integration. Indeed, for each substance, you can select and display the wavelength/illumination the substance has to be assigned to.
The main view is made of three main parts:
Profiles view (left)
Substances view (middle)
Tracks view (right)
When going to the Substance assignment tab, each unassigned substance is automatically assigned, based on the Rf required and ΔRf required values defined in the Definition tab. When several peaks are in the range on a given track, the highest peak is chosen. Then, Rf found and ΔRf found values are computed, respectively as the mean Rf value of the peaks found and as the minimal value possible containing all the peaks. The same applies when modifying manually the Rf or ΔRf values (see the next sections Profiles view and Substances view). In other words, the determination of the Rf found and ΔRf found values is semi-automatic.
The Profiles viewer shows the track profiles of the selected wavelength in the Overview section. Depending on the configurations made during the Integration, the peaks and the tracks are only detected and shown on the tracks selected (On switch) and within the Bounds range defined. The Profiles viewer is only in 2D mode here, but you can zoom in it to see the peaks in detail.
When zooming, the substance position overlay may not be placed correctly, go back to the default 1:1 ratio to see it correctly.
When a substance is selected, an overlay is displayed on the profiles viewer, corresponding to the ΔRf range around the Rf position found for the substance.
You can change the ΔRf of the substance by dragging one of the dashed lines or one of the triangles:
Each substance is displayed by a thumbnail (placed on the corresponding Rf):
The following actions are possible on each substance’s thumbnail:
- Change the Rf of the substance
By dragging the thumbnail.
- Select the used wavelength
By selecting a wavelength in the combo-box.
- Manually assign a peak to the substance
When toggled on, you can select one peak per track. You can also unassign a peak by clicking on it. When you have finished the assignment for all tracks, toggle off the button.
- Display the assignment
By clicking on the eye button the overlay on profiles view and tracks view is displayed. The column of the substance is also selected in the Substance Table.
Performing a manual assignment does not trigger the automatic computation of the Rf found and ΔRf found values.
Manually assigned peaks are lost when computing the Rf found and ΔRf found values.
Manual assignment gives you the choice to select any peak: it is possible to have two substances crossing across the plate, which may or may not be correct.
All active tracks are displayed. In case of a Visualizer step, the pseudo tracks coming from the profile generation are shown, not the image itself:
To the right of the track number, the type of sequence is displayed, for reference and for sample. The icons have tooltips displaying the application in details.
On each track, each detected peak is displayed by a small rectangle around the max. Rf position (peak start and end aren’t displayed).
The color of the peak rectangle corresponds to:
Grey: peak without an assigned substance
Green: peak with an assigned substance
Yellow: assigned peak of the selected substance
When a substance is selected, an overlay is displayed on all tracks, the white band showing the ΔRf found, and the dashed red line the Rf found value used for the substance.
A specific track (and its profile) can be selected by clicking on it. Multiple selection is not allowed.
The toolbar give access to some advanced functionalities:
Show all tracks: resets the selection of a specific track
Reset assignment: restart the automatic substance assignment
Add substance: you will be asked to click on a peak, a new substance will be automatically assigned to it