System Suitability Testing (SST)


SST tracks are defined in the Sample Sequence, column SST.


When one or more SST are defined, popup will remind the user to check the SST before working on evaluation, or exporting to comparison.

In this tab, you can define and validate SST.


SST Table

It is the definition of the tests, e.g. which substance should be at which Rf. Each SST definition has the following fields:

  • Substance: the substance name of the SST spot

  • Rf: the nominal Rf value of this SST

  • ΔRf: the authorized variation around the nominal Rf

  • Step: the step which should be used for checking

  • λ: wavelength or illumination which should be used for checking (only the ones available from the select step are visible)

  • Min. height: the minimum height of the peak detected at the Rf/ΔRf range with default integration parameters (corresponding to the default evaluation integration parameters when creating a new evaluation). You can let this field empty to indicate that the SST doesn’t check the peak height at all.

  • Status: the state of SST definition:

    • state-failed Failed

    • state-check Passed

    • state-checkmanual Manually marked as passed

    • state-notexecuted Not computed: click on the Check button (all the steps used in the SST definition should already be done)

  • Description

  • addsubstance Add an SST substance

  • deletesubstance Delete the currently selected SST substance definition

  • generateprofile Generate profiles for all track (from image data)


    • Deletes all output data (peaks, assignments, calibration functions and results) for evaluations based on a Visualizer step

    • Only available if the current analysis does not contain any locked evaluation based on a Visualizer step

  • check Check SST for all substances


    Check SST will automatically verify that each SST definition match a peak on each SST track.


Here you can select the illumination or wavelength of a specific HPTLC step you want to view. Clean plate data acquisition steps are not shown. For Visualizer steps, the profiles must be generated by using the Generate Profiles function in the analysis’ Data tab.


The selected illumination or wavelength in overview will not influence the automatic check.


This section is not available for visionCATS Methods.

SST View

In this section, the profile (on the left side) and the pseudo track (right side) is displayed for each track defined as SST, at the selected illumination/wavelength. In the middle, each substance is positioned relatively to its Rf and Rf window defined in the SST Table. You can check a substance to define it as Passed.

The main display has the following options:

  • It’s possible to display only one profile on the left side by selecting the corresponding pseudo track on the right side. In the toolbar, click on the first button to reset the selection.

  • In case of Visualizer profiles and tracks, the list of tracks can be displayed in gray scale or in RGB mode.

  • In order to better localize the position of the peaks, the toolbar includes a function to display the local maximum on each track in each substance window.

  • The zoom tool will allow to inhance the displayed separation (Rf axis). The peak forms are then also more visible.

For each SST definition, a substance banner would be displayed:



It is possible to manually mark as passed a SST defintion via the check box on the banner.


This section is not available for visionCATS Methods.