Instrument step parameters

Each step of a method/analysis appears in the Chromatography tab and can be configured by clicking on it. The Plate Layout step always appears first Though each instrument has its own set of parameters, they share common behaviors:

  • Default values are given to the different parameters in order to simplify the configuration for the user, meaning that you often won’t have to configure manually each step.

  • Instrument configuration windows have been designed to present the most important parameters first and to allow the user to access advanced parameters if needed (easy/expert paradigm).

  • Each instrument step has a Notes field and classic OK/Cancel buttons.

  • You can navigate back/forward through the analysis steps by using the corresponding buttons in the header of the step tooltips.

Here is the list of the different steps available:

Plate Layout

Define here the global parameters relative to the plate to be used and to the application.

../../../_images/plate_layout.png

Note

Be careful to configure the plate format according to the CAMAG instruments in use.

Note

When switching from/to Left/Center in the Track Assignment, the First track position X [mm] parameter is set.

CAMAG® HPTLC PRO Module APPLICATION

Define here the parameters relative to the execution of the HPTLC PRO Module APPLICATION.

../../../_images/proapp.png

In most cases, an APPLICATION module step requires a single sample solvent type. If vials must be applied with different parameters, several Sets must be defined. A new set can be added via the + on the left:

../../../_images/proappsets.png

Each Set is identified by an order and the sample solvent type name on which it is based. The popup allows to add, edit and remove sets.

When the step contains several sets, each applied vial must then be associated with a set in the Instructions panel (see Instruction display):

../../../_images/proappsetsinstructions.png

ATS 4

Define here the parameters relative to the execution of the ATS 4 instrument. The window provides default solvent types and filling/rinsing quality configurations.

../../../_images/ats4.png

Note

Rack positions are defined in the Track Assignment

Linomat 5

Define here the parameters relative to the execution of the Linomat 5 instrument (semi-automatic application).

../../../_images/linomat5.png

Manual application

Manual application step with any kind of manual application device:

../../../_images/nanomat.png

CAMAG® HPTLC PRO Module DEVELOPMENT

Define here the parameters relative to the execution of the HPTLC PRO Module DEVELOPMENT (single application).

../../../_images/prodev.png

ADC 2

Define here the parameters relative to the execution of the ADC 2 instrument (single application). The window provides some default development type parameters.

../../../_images/adc2.png

Note

When having several ADC 2 steps, the solvent front position can be defined here and not only in the Plate Layout parameters.

AMD 2

Define here the parameters relative to the execution of the AMD 2 (multiple applications). The General tab handles the configuration of the bottles and the instrument itself.

../../../_images/amd2_general.png

The Gradients tab contains the configuration of the gradients steps, with auto-fill options.

../../../_images/amd2_gradient.png

Note

The Graphic tab consists in a visual representation of the Gradients tab.

Chamber

Define here the parameters relative to the execution of a development in a glass development chamber.

../../../_images/chamber.png

CAMAG® HPTLC PRO Module DERIVATIZATION

Define here the parameters relative to the execution of the HPTLC PRO Module DERIVATIZATION.

../../../_images/proder.png

Note

Heating before spraying consists in the pre-heating of the HPTLC plate.

Derivatizer

Define here the parameters relative to the execution of the Derivatizer instrument.

../../../_images/derivatizer.png

Immersion

Define here the parameters relative to the execution of a derivatization by dipping.

../../../_images/derivatization_dip.png

Spraying

Define here the parameters relative to the execution of a derivatization by spraying.

../../../_images/derivatization_spray.png

TLC Visualizer

Define here the parameters relative to the execution of a TLC Visualizer documentation step. A visualizer step can contain up to 5 images (called Illuminations), with the possibility to repeat them in a sequence.

../../../_images/visualizer.png

Note

The Documentation step label field can facilitate the identification of documentation step data when using them.

Note

The Visualizer High-Res documentation package option is required to take Enhanced images.

TLC Scanner 4

Define here the parameters relative to the execution of a TLC Scanner 4 documentation step.

../../../_images/scanner4.png

A scanner step can be used to perform a single-wavelength, multi-wavelength or spectrum measurement. When choosing multi-wavelength, it’s possible to define a pattern to apply:

../../../_images/scanner4_mwl.png

Note

An Advanced mode is also available, where the measurement mode, lamp and filter can be set independently for each wavelength.

When choosing spectrum, the range and some specific parameters have to be set:

../../../_images/scanner4_spectrum.png

Note

The Documentation step label field can facilitate the identification of documentation step data when using them.

Note

The Multi Wavelength Scanning option is required to use the multi-wavelength mode.

Note

The Spectrum Scanning option is required to use the spectrum mode.

TLC Scanner 3

Define here the parameters relative to the execution of a Scanner 3 documentation step. The parameters are the same as the ones used for a Scanner 4 step, except that the maximum wavelength for a Scanner 3 step is 800 nm instead of 900 nm.

../../../_images/scanner3.png

Common features

After importing an analysis or method, it is possible that the global list item is missing:

../../../_images/unimported_item_warning.png

In such case, it should manually be added, with the needed parameters.